Invitrogen™

CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for자세히 알아보기

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features of the CellsDirect™ One-Step qRT-PCR Kit include:
• Efficiency—Offers significant time and cost savings for increased throughput
• Convenience—Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
• Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
• Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for Your qRT-PCR Application
CellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.

CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g. TaqMan™) or LUX™ fluorogenic primers.

Note: This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit (11753-500), containing a separate tube of ROX™ Reference Dye, is also available.

사양

검출 방법Primer-probe

반응 속도Fast or Standard

용도(장비)7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system

제품라인CellsDirect™, Platinum™, SuperScript™

수량100 reactions

용도(애플리케이션)RT-PCR

농도2X

반응 수100 Reactions

샘플 종류Cells

Unit SizeEach