Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), APC-eFluor 780, eBioscience
마우스 CD4 분자를 인식하는 RM4-5 단클론 항체로, APC-eFluor 780 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.125 µg/test로 사용 가능합니다. 633 nm 레이저에 의해 여기되며 780 nm에서 방출됩니다. 4°C 어둠 속 보관 권장.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), APC-eFluor 780, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- View 206 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RM4-5 |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272219 |
Additional Formats Available
- Unconjugated, Alexa Fluor 532, 561, 647, 660, 700
- APC, Biotin, Brilliant UV Series, Brilliant Violet Series
- eFluor 450, 506, 570, 615
- FITC, PE, PE-Cyanine Series, PerCP Series
- Super Bright Series, NovaFluor Series
- Request custom conjugation
Product Specific Information
Description:
The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulations of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is essential for T cell development and function. Binding of RM4-5 is blocked by GK1.5.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.125 µg/test (100 µL final volume). Optimize empirically for best performance.
Fluorochrome Properties:
- Excitation: 633–647 nm
- Emission: 780 nm
- Laser: Red laser
- Light sensitivity: Protect from light due to photo-induced oxidation sensitivity.
- Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD4 antigen is a co-receptor for MHC class II recognition and HIV binding. It is expressed on T helper cells, monocytes, macrophages, and dendritic cells. CD4 facilitates T-cell activation through association with the T-cell receptor complex and tyrosine kinase Lck. Expression is found in thymus, lymph nodes, spleen, and other tissues. CD4 may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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