Thermo Fisher Scientific Phospho-Vimentin (Ser39) Polyclonal Antibody
Phospho-Vimentin (Ser39) 폴리클로날 항체로 Western blot, IHC, ELISA에 적합. 인간, 마우스, 랫트 반응성. Rabbit IgG 기반, 액상형, 친화 크로마토그래피 정제. 세포 골격 단백질 Vimentin의 인산화 상태 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S39 of human VIM (NP_0033712) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.56 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901845 |
Product Specific Information
- Positive Samples: HeLa, NIH/3T3
- Immunogen sequence: TYSLG
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. During development, vimentin is replaced by tissue-specific IFPs. It also plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangements that enhance cholesterol transport to mitochondria, promoting steroid synthesis.
주의사항
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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