Thermo Fisher Scientific HP1 alpha Recombinant Mouse Monoclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Immunogen

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Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532847

Product Specific Information

730046 is a biotinylated Fab fragment targeting HP1 alpha.

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.

Target Information

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double- primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.