Thermo Fisher Scientific DDX3 Polyclonal Antibody
Rabbit polyclonal antibody against human DDX3, validated for WB, IHC, and IP. Recognizes residues 1–50 of DDX3. Supplied as liquid, 0.2 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 (DDX3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| Shelf Life | 1 year from date of receipt |
Target Information
The DDX3 protein is a member of the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and ATP-dependent RNA helicase activity. It exhibits high levels of RNA-independent ATPase activity, and unlike most DEAD-box helicases, its ATPase activity is stimulated by both RNA and DNA.
DDX3 plays roles in both the nucleus and cytoplasm:
- Nuclear functions: transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export
- Cytoplasmic functions: translation, cellular signaling, and viral replication
Misregulation of DDX3 has been implicated in tumorigenesis. The gene has a paralog on the Y chromosome (DDX3Y), and pseudogenes with similarity to both DDX3 and DDX3Y are found on chromosome 4 and the X chromosome. Alternative splicing generates multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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