Thermo Fisher Scientific DDX3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human DDX3 protein for WB, IHC, and IP applications. High specificity with antigen affinity purification. Suitable for human and mouse samples. Stored at 4°C, shipped on wet ice.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
The protein encoded by this gene is a member of the large DEAD-box protein family, defined by the conserved Asp-Glu-Ala-Asp (DEAD) motif, and exhibits ATP-dependent RNA helicase activity. This protein shows high RNA-independent ATPase activity and is stimulated by both RNA and DNA, unlike most DEAD-box helicases. It functions in both nucleus and cytoplasm, contributing to transcriptional regulation, mRNP assembly, pre-mRNA splicing, mRNA export, translation, cellular signaling, and viral replication. Misregulation of this gene is linked to tumorigenesis. A paralog exists on the Y chromosome, and pseudogenes are found on chromosome 4 and the X chromosome. Alternative splicing produces multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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