Thermo Fisher Scientific CD2 Monoclonal Antibody (RM2-5), FITC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 5 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Biotin
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• Request custom conjugation

Product Specific Information

Description:
The RM2-5 monoclonal antibody reacts with the mouse CD2 molecule, an approximately 50–55 kDa cell surface receptor expressed by all mouse lymphocytes. Expression of the CD2 antigen in the mouse differs from that of the human in that B lineage cells in the mouse from the pre-B cell stage to mature B cells express CD2. CD2 is a ligand for CD48 in the mouse and is involved in adhesion and activation of T cells.

Applications Reported:
The RM2-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. Recommended usage ≤ 0.25 µg/test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.

Fluorescence Characteristics:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD2 (LFA-2) is a monomeric surface antigen (MW range 45–58 kDa) of the human T-lymphocyte lineage expressed on all peripheral blood T cells. It is one of the earliest T-cell markers, present on >95% of thymocytes, and also found on some NK cells but not B lymphocytes. Monoclonal antibodies against CD2 inhibit rosette formation with sheep erythrocytes, indicating CD2’s role as an erythrocyte receptor. CD2–CD58 interactions stabilize adhesion between T cells and antigen-presenting cells. Low affinity in solution is compensated by high avidity at the cell-cell interface. T cell activation increases CD2 expression and mobility, enhancing adhesion. CD2 deficiency can reduce intestinal inflammation and help control infection. Diseases associated with CD2 dysfunction include penile squamous cell carcinoma and immune deficiency due to thymus absence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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