Thermo Fisher Scientific MiTF Recombinant Rabbit Monoclonal Antibody (JF100-01)
MiTF 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Flow cytometry 등에 사용 가능. 높은 특이성과 일관성, 동물 유래 성분 없음. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | View 2 publications |
| Flow Cytometry (Flow) | 1:50–1:100 | View publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF100-01 |
| Immunogen | Recombinant protein within Human MiTF aa 353–520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809831 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Clones are screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
MiTF (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor involved in pigmentation, mast cell, and bone development.
Mutations in MiTF cause Waardenburg Syndrome type II in humans.
In mice, loss of MiTF leads to depigmentation, osteopetrosis, and defects in natural killer and mast cells.
Two isoforms exist, differing by 66 amino acids at the N-terminus:
- Shorter forms (52–56 kDa): expressed in melanocytes
- Longer forms (60–70 kDa): expressed in osteoclasts, B16 melanoma cells, mast cells, and heart
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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