Thermo Fisher Scientific HGF Polyclonal Antibody
HGF 단백질을 인식하는 Thermo Fisher Scientific의 염소 폴리클로날 항체로, Western blot, IHC, 중화 실험에 적합합니다. 인간 시료에 반응하며, 비결합형 액상 형태로 제공됩니다. 세포 성장, 이동, 형태형성 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (IHC) | 20 µg/mL |
| Neutralization (Neu) | 0.5 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant human hepatocyte growth factor (rhHGF), expressed in insect cell line Sf21 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2118758 |
Product Specific Information
- Recommended positive controls: cervical lesions
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Hepatocyte growth factor (HGF) regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade upon binding to the proto-oncogenic c-Met receptor. It is secreted by mesenchymal cells and functions as a multifunctional cytokine primarily on epithelial cells. HGF promotes mitogenesis, cell motility, and matrix invasion, playing key roles in angiogenesis, tumorigenesis, and tissue regeneration.
HGF is secreted as a single inactive polypeptide and cleaved by serine proteases into a 69-kDa alpha-chain and a 34-kDa beta-chain, forming an active heterodimer via a disulfide bond. The protein belongs to the plasminogen subfamily of S1 peptidases but lacks protease activity. Alternative splicing produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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