Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), PE, eBioscience
인간 및 영장류 FOXP3 단백질을 인식하는 단클론 항체로, CD4+CD25+ 조절 T세포 분석에 적합합니다. PE 형광 표지로 유세포분석(Flow Cytometry)과 IHC에 사용됩니다. 236A/E7 클론으로 검증된 신뢰성 높은 항체입니다.
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- 12-4777-4x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), PE, eBioscience
Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | 59 publications |
| Luminex (LUM) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rhesus monkey |
| Published Species | Baboon, Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 236A/E7 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1944448 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- eFluor 450
- eFluor 570
- eFluor 660
- FITC
- PE-Cyanine5
- PE-Cyanine7
- Custom conjugation available upon request
Product Specific Information
The 236A/E7 antibody reacts with human Foxp3 protein (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3 (49–55 kDa) is a transcriptional regulator of the forkhead/winged-helix family, identified as the gene defective in scurfy (sf) mice. High expression of Foxp3 mRNA is observed in CD4+CD25+ regulatory T cells (Treg), and ectopic expression in CD4+CD25− cells induces a Treg phenotype.
Intracellular staining and flow cytometric analysis of human PBMCs using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523) reveals staining of the CD4+CD25^bright population.
The epitope recognized by 236A/E7 differs from that of PCH101 (Product #72-5776-40). This antibody also recognizes rhesus macaque, sooty mangabey, and cynomolgus macaque.
Applications Reported: Intracellular staining, flow cytometry, and IHC.
Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometry using the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00).
Recommended usage: 5 µL (0.25 µg) per test in 100 µL final volume. Applicable for 10^5–10^8 cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long and expressed highly in CD25+CD4+ regulatory T cells, low in CD4+CD25− cells, and absent in CD4−CD8+ cells. FOXP3 acts as a master regulatory gene and a specific marker for Treg cells. Mutations cause scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. Expression of FOXP3 in cancer cells is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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