Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor 488, eBioscience
Alexa Fluor 488으로 결합된 RA3-6B2 클론의 CD45R(B220) 단클론 항체로, 마우스 및 인간 시료에서 B세포 표지에 사용됩니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, 499/520 nm 형광 특성을 가집니다. 4°C 암소 보관.
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Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 30 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | 20 µg/mL | 2 |
| Immunocytochemistry (ICC/IF) | - | 8 |
| Flow Cytometry (Flow) | 0.5 µg/test | 69 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RA3-6B2 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469906 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Yellow series
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine series
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series
- NovaFluor Blue, Red, Violet, Yellow series
- Request custom conjugation available
Product Specific Information
The RA3-6B2 monoclonal antibody reacts with the exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. The CD45R/B220 epitope is mainly expressed by B cell lineage from early Pro-B to mature B cells, but also on certain activated T cells, LAK cells, NK progenitors, and lpr/lpr mutant mouse T cells.
Applications Reported:
Used for immunohistochemical staining (frozen and paraffin sections) and flow cytometric analysis.
Applications Tested:
- Flow Cytometry: ≤ 0.5 µg/test
- IHC (Frozen): ≤ 20 µg/mL
- IHC (Paraffin): ≤ 20 µg/mL (low pH antigen retrieval recommended)
Antibody should be titrated for optimal assay performance.
Fluorescence Characteristics:
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Filtered through 0.2 µm post-manufacturing.
Target Information
CD45R (PTPRC) belongs to the protein tyrosine phosphatase (PTP) family, which regulates cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 is expressed in hematopoietic cells and is essential for T- and B-cell receptor signaling. It exists in multiple isoforms via alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45 regulates Src-family kinases, contributes to lymphocyte development, antigen signaling, and integrin-mediated survival pathways. Dysfunction in CD45 is associated with Severe Combined Immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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