Thermo Fisher Scientific GCDFP-15 Monoclonal Antibody (PIP, 1571)
GCDFP-15 단백질을 인식하는 마우스 모노클론 항체로, 인간 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능하며, 단백질 A/G로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific GCDFP-15 Monoclonal Antibody (PIP, 1571)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PIP, 1571 |
| Immunogen | Recombinant human GCDFP-15 protein fragment (around aa 41–146) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Gross cystic disease of the breast is a benign premenopausal disorder characterized by cyst formation due to excessive apocrine secretions. The cystic fluid contains several glycoproteins, including the unique 15 kDa monomer protein GCDFP-15 (Gross Cystic Disease Fluid Protein-15).
GCDFP-15 is found in apocrine epithelia, lacrimal, ceruminous, and Moll’s glands, as well as in serous cells of submandibular, tracheal, bronchial, sublingual, and minor salivary glands. GCDFP-15 and prostate-specific antigen are co-expressed in androgen receptor-positive breast tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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