Thermo Scientific™

pCMV-Gaussia-Dura Luc Vector for Luciferase Assays

The Thermo Scientific pCMV-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia자세히 알아보기

The Thermo Scientific pCMV-Gaussia_-Dura Luc vector is a derivative of pMCS-_Gaussia_-Dura Luc. It contains a mutant form of the _Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the strong Cytomegalovirus (CMV) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pCMV-_Gaussia_-Dura Luc vector:

• Strong (CMV) constitutive promoter for co-transfection and normalization
• Naturally-secreting _Gaussia_-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

_Gaussia_-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

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사양

설명pCMV-Gaussia-Dura Luc Vector

유형Luc Vector

클로닝 방법Restriction Enzyme/MCS

배달 유형Transfection

수량10 μg

리포터 유전자Gaussia Dura Luciferase

선택 제제(진핵)Puromycin

용도(애플리케이션)Reporter Assays

프로모터CMV

Unit SizeEach