Thermo Scientific™

pTK-Gaussia-Dura Luc Vector for Luciferase Assays

The Thermo Scientific pTK-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia자세히 알아보기

The Thermo Scientific pTK-Gaussia_-Dura Luc vector is a derivative of pMCS-_Gaussia_-Dura Luc. It contains a mutant form of the _Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the weak Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pTK-_Gaussia_-Dura Luc vector:

• Weak (TK) constitutive promoter for co-transfection and normalization
• Naturally-secreting _Gaussia_-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

_Gaussia_-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

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pMCS-Gaussia-Dura Luc Vector for Luciferase Assays
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사양

설명pTK-Gaussia-Dura Luc Vector

유형Luc Vector

클로닝 방법Restriction Enzyme/MCS

배달 유형Transfection

수량10 μg

리포터 유전자Gaussia Dura Luciferase

선택 제제(진핵)Puromycin

용도(애플리케이션)Reporter Assays

프로모터TK

Unit SizeEach