Thermo Fisher Scientific pTK-Gaussia-Dura Luc Vector for Luciferase Assays
약한 TK 프로모터 기반의 루시퍼레이스 벡터로 실험 간 변동 보정에 적합. Gaussia-Dura 루시퍼레이스 유전자를 포함하여 안정적 발광 신호 제공. MCS로 다양한 조절 요소 클로닝 가능. 퓨로마이신 및 암피실린 선택 마커 포함.
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Thermo Scientific™ pTK-Gaussia-Dura Luc Vector for Luciferase Assays
The Thermo Scientific pTK-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene, conferring improved bioluminescent signal stability compared to the native luciferase, under the control of the weak Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter.
This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.
Features
- Weak (TK) constitutive promoter for co-transfection and normalization
- Naturally secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
- Multiple cloning site (MCS) for flexible transfer of regulatory elements between plasmids
- Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) to minimize background by reducing transcriptional read-through
- Dual selection markers: puromycin (Pur) for mammalian cells and ampicillin (Amp) for bacterial selection
- High-copy pUC bacterial DNA replication origin
Description
Gaussia-Dura luciferase (~20 kDa) is a secreted protein that allows measurement of reporter activity in media for real-time assays as well as in cell lysates.
The parent pMCS vector includes a multiple cloning site for promoter cloning to study regulatory potential.
These vectors are subject to a limited use label license.
Related Products
- pMCS-Gaussia-Dura Luc Vector for Luciferase Assays
- pCMV-Gaussia-Dura Luc Vector for Luciferase Assays
Specifications
| 항목 | 내용 |
|---|---|
| 설명 | pTK-Gaussia-Dura Luc Vector |
| 유형 | Luc Vector |
| 클로닝 방법 | Restriction Enzyme / MCS |
| 배달 유형 | Transfection |
| 수량 | 10 μg |
| 리포터 유전자 | Gaussia Dura Luciferase |
| 선택 제제 (진핵) | Puromycin |
| 용도 (애플리케이션) | Reporter Assays |
| 프로모터 | TK |
| Unit Size | Each |
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