Thermo Fisher Scientific pTK-Gaussia-Dura Luc Vector for Luciferase Assays

Thermo Scientific™ pTK-Gaussia-Dura Luc Vector for Luciferase Assays

The Thermo Scientific pTK-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene, conferring improved bioluminescent signal stability compared to the native luciferase, under the control of the weak Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter.
This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features

• Weak (TK) constitutive promoter for co-transfection and normalization
• Naturally secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) for flexible transfer of regulatory elements between plasmids
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) to minimize background by reducing transcriptional read-through
• Dual selection markers: puromycin (Pur) for mammalian cells and ampicillin (Amp) for bacterial selection
• High-copy pUC bacterial DNA replication origin

Description

Gaussia-Dura luciferase (~20 kDa) is a secreted protein that allows measurement of reporter activity in media for real-time assays as well as in cell lysates.
The parent pMCS vector includes a multiple cloning site for promoter cloning to study regulatory potential.

These vectors are subject to a limited use label license.

Related Products

• pMCS-Gaussia-Dura Luc Vector for Luciferase Assays
• pCMV-Gaussia-Dura Luc Vector for Luciferase Assays

Specifications