Thermo Scientific™

Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

The Thermo Scientific Pierce Renilla-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red자세히 알아보기

The Thermo Scientific Pierce Renilla_-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular _Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

Features of the _Renilla_-Firefly Luciferase Dual Assay Kit:

• Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
• Sensitive—measure Green Renilla and red firefly luciferase activities in the same sample
• Fast—no quenching step required, unlike in traditional sequential dual assays
• Multiplex—capable of quantitating two cellular activities in the same sample(s)

The Pierce Renilla-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Green Renilla and Red Firefly luciferase activity. Green Renilla luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animation video below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Includes:
Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin

Requires:
Green Renilla and Red Firefly luciferase reporters; Filter sets at 525nm±20nm for Green Renilla Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Applications:
• Study two regulatory elements at the same time
• Monitor two signaling pathways simultaneously
• Enable studying more than one target per screen (e.g. off-target effects)

Green Renilla produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent signal (λmax= 535nm) produced by Green Renilla luciferase protein results from the oxidation of coelenterazine. The second assay uses red firefly luciferase, which is a mutant form of the Japanese firefly luciferase Luciola cruciata. This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP.

More Product Data
• Simultaneous dual-emission detection of luciferase reporter assays

Related Products
Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

사양

유형Assay Kit

타겟Luciferase, Firefly Luciferase, Green Renilla Luciferase

충분100 Dual Luciferase Assays

수량100 Reaction Kit

어세이Reporter Enzyme, Luciferase Reporter Assay

호환 셀Mammalian Cells

검출 방법Bioluminescence

용도(장비)Luminometer (Microplate)

형식384-well plate, 96-well plate

라벨 유형Enzyme Labeled

기질 특성Chemical Substrate

기술Enhanced Chemiluminescence

기질 유형Luciferase Substrate

제품라인Pierce™

기질Coelenterazine, D-Luciferin

Unit SizeEach