Thermo Fisher Scientific CD27 Monoclonal Antibody (OTI8E5)

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. Members of this family regulate cell proliferation and death.
CD27 and its ligand CD70 are primarily expressed on lymphocytes. CD27 expression increases upon antigen receptor ligation, while CD70 expression requires co-stimulatory or pro-inflammatory signals.
CD27 forms a disulfide-linked homodimer found on mature thymocytes, peripheral blood T cells, and a subset of B cells. Activation of T cells via the TCR-CD3 complex leads to increased CD27 expression and release of soluble CD27 (sCD27, 28–32 kDa), detectable in plasma, urine, or spinal fluid.
Soluble CD27 serves as a prognostic marker for acute and chronic B cell malignancies. RgpA, a cysteine protease, can degrade CD27 and counteract its activation. CD27-binding protein (SIVA) is a proapoptotic protein involved in CD27-mediated apoptosis.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.