
Thermo Fisher Scientific BAFF Recombinant Rabbit Monoclonal Antibody (182)
Human BAFF 단백질을 인식하는 Thermo Fisher Scientific의 Recombinant Rabbit Monoclonal Antibody (Clone 182). Western blot, Flow cytometry, Immunoprecipitation에 적합. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 제작됨.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 182 |
| Immunogen | Recombinant Human BLyS/TNFSF13B/BAFF protein (Ala134–Leu285) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785449 |
Additional Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human BLyS/TNFSF13B/BAFF.
Target Information
BAFF is a membrane-bound cytokine expressed by B cell lineage cells. The soluble form binds to receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. It is a potent activator promoting B cell proliferation and differentiation. BAFF is also expressed by T lymphocytes and contributes to T cell activation and survival. Isoforms representing alternatively spliced and glycosylated variants have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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