Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), Alexa Fluor 488, eBioscience
마우스 IL-13을 인식하는 단클론 항체로, Alexa Fluor 488 형광 표지되어 있습니다. Flow cytometry, IHC, ELISA, 중화 실험에 사용 가능합니다. 0.25 µg/test로 최적화되어 있으며, 4°C 암소 보관 권장. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 |
| Flow Cytometry (Flow) | 0.25 µg/test | 47 |
| ELISA | - | 1 |
| Neutralization | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio13A |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2016639 |
Additional Formats Available
- Unconjugated
- APC-eFluor 780
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The eBio13A antibody reacts with mouse IL-13, a cytokine produced mainly by Th2 cells and antigen-primed CD8 T cells. IL-13 is involved in allergic inflammation, parasitic response, and tumor regulation, showing either inhibitory or stimulatory activity depending on the context. In humans, IL-13 contributes to isotype switching in B cells and downregulates macrophage activity by reducing pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12).
Applications Reported:
Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Validated for intracellular staining and flow cytometric analysis of Th2-polarized CD4 cells. Recommended use ≤ 0.25 µg/test (in 100 µL final volume). Cell number per test may range from 10⁵–10⁸, to be optimized empirically. Antibody titration recommended for best performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by downregulating macrophage activity, reducing pro-inflammatory cytokine production, and contributing to allergen-induced asthma through IgE-independent mechanisms. IL-13 also regulates B cell maturation and differentiation, upregulates CD23 and MHC class II expression, and promotes IgE isotype switching. The IL-13 gene is located within a cytokine gene cluster on chromosome 5q, near IL4, IL3, IL5, and CSF2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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