Thermo Fisher Scientific ERK1/ERK2 Monoclonal Antibody (9B3)
ERK1/ERK2 단백질 검출용 Thermo Fisher Scientific의 Mouse Monoclonal Antibody. Western blot, ICC/IF, Flow Cytometry에 적합. HepG2 세포를 양성 대조군으로 권장. 세포 신호 전달 및 MAPK 경로 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 3–5 µg/1×10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9B3 |
| Immunogen | Synthetic peptide, KLH coupled, corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (Erk1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_1075547 |
Product Specific Information
A suggested positive control for this product is HepG2 cells.
Target Information
ERK1 and ERK2 are widely expressed and play critical roles in regulating meiosis, mitosis, and postmitotic functions in differentiated cells. They are activated by various stimuli including growth factors, cytokines, viral infection, and G protein-coupled receptor ligands. Upon activation, Ras interacts with Raf kinase, which phosphorylates MEK1/2. MEK1/2 subsequently phosphorylates ERK1/2 on tyrosine/threonine residues. Activated ERKs then phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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