Thermo Fisher Scientific iNOS Monoclonal Antibody (M398)
iNOS 단백질을 특이적으로 인식하는 Mouse monoclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 ICC/IF에 적합하며, Protein A 정제 및 PBS/BSA/glycerol buffer로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M398 |
| Immunogen | Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942707 |
Product Specific Information
The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFNγ and LPS, or J774A.1 cells treated with LPS only.
Target Information
iNOS (Inducible Nitric Oxide Synthase, NOS) is an enzyme responsible for the production of nitric oxide (NO), a gaseous free radical that mediates various cellular processes such as vasorelaxation, neurotransmission, and cytotoxicity.
NO production is catalyzed by the NOS enzyme family, which includes three isoforms: neuronal NOS (nNOS, type I), endothelial NOS (eNOS, type III), and inducible NOS (iNOS, type II).
iNOS expression is induced by cytokines (e.g., IFNγ, TNF, IL-1, IL-2) and lipopolysaccharides (LPS), leading to increased mRNA, protein, and enzymatic activity. It is expressed in macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
After cytokine induction, iNOS shows delayed but sustained NO production.
Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Dysfunction of iNOS has been associated with diseases such as achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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