Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), PE, eBioscience
마우스 NKp46(CD335)을 인식하는 단클론 항체로, NK 세포 표지 및 분석에 적합합니다. PE 형광 표지로 유세포분석에 최적화되어 있으며, 다양한 마우스 계통에서 비특이적 염색 없이 사용 가능합니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 46 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29A1.4 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_996682 |
Available Conjugate Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail, expressed uniquely on NK cells, including immature NK cells (DX5-, CD3-), allowing discrimination from NKT cells (NKp46+, CD3-).
Unlike many NK markers that also stain NKT cells, staining with 29A1.4 is not strain-specific and has been shown on C57Bl/6, SJL, CBA/CA, and BALB/C strains. NKp46 is involved in NK cell-mediated lysis of tumor and pathogen-infected cells.
The 29A1.4 antibody activates NK cells in vitro without depleting them in vivo.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes
- Suggested Use: ≤ 0.5 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser Compatibility: Blue, Green, Yellow-Green Lasers
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor that enhances NK cell-mediated tumor cell lysis. It contains two Ig-like domains similar to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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