Thermo Fisher Scientific CD44/HCAM Std. Recombinant Rabbit Monoclonal Antibody (HCAM, 2875R)

Applications

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration. It participates in various cellular functions including lymphocyte activation, recirculation, and homing. CD44 expression may be up-regulated in certain carcinomas and is speculated to be related to metastatic potential. It is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate CD44 expression upon stimulation. Transcripts undergo complex alternative splicing, resulting in many distinct isoforms. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 may also be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.