Thermo Fisher Scientific Beta-catenin Monoclonal Antibody (OTI1E10), TrueMAB
Beta-catenin 단백질을 검출하기 위한 Monoclonal Antibody로, Western blot, IHC, ICC, Flow Cytometry에 최적화됨. Human 및 Dog 시료에 반응하며, IgG2a 동종형의 Mouse 유래 항체. 동결건조 형태로 제공되며, PBS buffer에 8% trehalose 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E10 |
| Immunogen | Full length human recombinant protein of human CTNNB1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Beta-catenin is a key adherens junction (AJ) protein involved in cell-cell adhesion and epithelial layer maintenance. It interacts with E-cadherin and alpha-catenin to anchor the E-cadherin complex to the actin cytoskeleton.
It also associates with signaling pathways including tyrosine kinases, phosphatases, and Wnt/Wingless, influencing morphogenesis and cell proliferation.
Beta-catenin expression is observed in hair follicle cells and epithelial tissues, with pathological roles noted in cancers such as colorectal, ovarian, and medulloblastoma.
In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors to promote genes like cyclin D and c-myc, contributing to tumorigenesis in various solid tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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