Thermo Fisher Scientific NUMB Recombinant Rabbit Monoclonal Antibody (14H45L9)
NUMB 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot 및 Immunoprecipitation에 적합. 높은 특이성과 민감도, 로트 간 일관성 보장. 인간 반응성으로, 단기 4°C 보관 및 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 1 publication |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 14H45L9 |
| Immunogen | Protein corresponding to human NUMB [aa460-aa635] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2762405 |
Product Specific Information
This antibody is predicted to react with Monkey, Horse, and Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells.
Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex.
NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca²⁺ channel (VGCC) inhibitor or by Ca²⁺ chelator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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