Thermo Fisher Scientific Cdc25C Polyclonal Antibody
Rabbit polyclonal antibody against human CDC25C for WB, IHC, and ICC applications. Recognizes recombinant CDC25C protein; useful for cell cycle and mitosis studies. Supplied as liquid, 1 mg/mL, unconjugated; store at 4°C or -20°C. For research use only.
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Thermo Fisher Scientific Cdc25C Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDC25C. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735617 |
Product Specific Information
- Positive Control: Raji, Raw264.7, C2C12, PC-12, Rat2
- Predicted Reactivity: Pig (94%), Chimpanzee (97%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
M-phase inducer phosphatase 3 is an enzyme encoded by the CDC25C gene in humans. This gene is highly conserved during evolution and plays a key role in regulating cell division. The encoded protein is a tyrosine phosphatase belonging to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants have been described, though the full-length nature of many remains unknown.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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