Thermo Fisher Scientific Zika Virus NS1 Monoclonal Antibody (EA23)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:50

-

ELISA (ELISA)

1:20-1:200

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

EA23

Immunogen

recombinant Zika Virus NS1 protien

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2633554

Product Specific Information

MA5-24582 does not cross react with the following viruses: Dengue 1-4, TBE virus, West Nile virus, Yellow Fever virus, Japanese Encephalitis virus and Chikungunya virus.

Target Information

As a member of the Flaviviridae family, ZIKV has a single positive sense RNA genome, which is initially translated as a single polyprotein which is then cleaved post-transitionally into three structural proteins (C, PrM or M, and E) and seven nonstructural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5)(1). The glycosated NS1 forms a homodimer inside the cells and is necessary for viral replication and late in infection (2). NS1 is also secreted into the extracellular space as a hexameric lipoprotein particle, which assists the virus in immune evasion and pathogenesis by interacting with components from both innate and adaptive immune systems as well as other host factors (1). Reference 1) Song, H.; Qi, J.; Haywood, J.; Shi, Y.; Gao, G.F. (2016). Nat. Struct. Mol. Biol. 23, 456-458. 2) Suthar, M.S., Diamond, M.S. & Gale, M. Jr. (2013). Nat. Rev. Microbiol. 11, 115-128.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.