Thermo Fisher Scientific Phospho-Stat4 (Tyr693) Recombinant Rabbit Monoclonal Antibody (Stat4Y693-F6), APC
Phospho-Stat4 (Tyr693) Rabbit Monoclonal Antibody, APC 형광 표지로 Flow Cytometry에 최적화된 제품. HEK293 세포 발현 시스템 기반, IL-12 신호 경로 연구 및 STAT4 인산화 분석에 적합. 2–8°C 보관, Wet ice 배송.
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Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Stat4Y693-F6 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr693 of human phospho Stat4 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Storage Conditions | 2–8°C |
| Shipping Conditions | Wet ice |
Additional Formats
Target Information
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 can be tyrosine phosphorylated by Jak1 or Jak2 and forms homodimers and heterodimers with related STAT family members. Phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and serine phosphorylation is required for maximal transcriptional activity.
STAT4 expression is restricted to the thymus, spleen, and testis. It is activated by interleukin 12 (IL-12), which is essential for T-cell independent induction of IFN-gamma and Th1 response development. STAT4-deficient mice show impaired IL-12-mediated Th1 development and enhanced Th2 differentiation. IFN-α/β activation of STAT4 is required for IFN-γ production during viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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