Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.125 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The eBio5E10 monoclonal antibody reacts with human CD90 (Thy-1), a 25–35 kDa receptor expressed on thymocytes, CD34⁺ prothymocytes, hematopoietic stem cells, neurons, and subsets of fetal liver, cord blood, and bone marrow cells. CD90 regulates adhesion and signal transduction in T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated on HEL cells. Recommended use at 5 µL (0.125 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.
• Fluorochrome: Brilliant Ultra Violet™ 563 (BUV563), emits at 564 nm, for UV (355 nm) laser-equipped cytometers.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal signal loss.
• Excitation / Emission / Laser: 350 nm / 564 nm / Ultraviolet Laser

Note: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD90 (Thy-1) is a GPI-linked glycoprotein belonging to the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. CD90 functions in cell-cell and cell-ligand interactions, particularly during synaptogenesis. It serves as a marker for various stem cells and neuronal axons. Dysregulation of CD90 is associated with diseases such as nasopharyngeal carcinoma and thymoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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