Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 11 publications 11 publications

Flow Cytometry (Flow)

Assay-Dependent

View 21 publications 21 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16B1

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• PE
• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2864993

Product Specific Information

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), microscopy, and immunocytochemistry without fixation.

Applications Tested: This 16B1 antibody has been tested by immunoblotting Human Umbilical Vein Endothelial Cell (HUVEC) lysates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.