Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), eBioscience
마우스 CD152(CTLA-4)를 인식하는 UC10-4B9 단클론 항체로, Flow Cytometry, IP, Functional Assay에 활용 가능. 비결합형 포맷이며, 고순도(>90%)로 정제됨. CTLA-4의 세포 내 및 표면 발현 분석에 적합. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 39 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UC10-4B9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864997 |
Additional Formats
- APC (Cat. #17-1522-82)
- Biotin (Cat. #13-1522-82)
- Brilliant UV 737 (Cat. #367-1522-82)
- PE (Cat. #12-1522-82)
- PE-Cyanine7 (Cat. #25-1522-82)
- PE-eFluor 610 (Cat. #61-1522-82)
- Custom conjugation available upon request
Product Specific Information
The UC10-4B9 monoclonal antibody reacts with mouse CD152 (CTLA-4), a protein structurally similar to CD28. CTLA-4 is expressed at low levels on activated T cells and binds B7 family members CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28. While CD28 acts as a potent costimulator, CTLA-4 inhibits T cell activation by restricting IL-2 secretion and proliferation.
Due to intracellular localization of CTLA-4, complete detection may require assessment of both surface and intracellular expression.
Applications Reported: Flow cytometry, immunoprecipitation, and in vitro functional studies (use Functional Grade purified UC10-4B9, Product #16-1522, for functional assays).
Applications Tested: Tested by flow cytometric analysis of resting and activated mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Optimal titration required per assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor mainly expressed on CD4+ T cells. It binds CD80 and CD86 with higher affinity than CD28 but delivers inhibitory signals, suppressing T cell activation. Intracellular CTLA4 is also found in regulatory T cells, contributing to their suppressive function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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