Thermo Fisher Scientific SMURF2 Recombinant Rabbit Monoclonal Antibody (8O7F0)
SMURF2 단백질을 표적하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. Human 및 Mouse 반응성. 고순도 Affinity Chromatography 정제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:800 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 400–600 µg extracts |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8O7F0 |
| Immunogen | Recombinant fusion protein corresponding to amino acids 201–290 of human SMURF2 (Q9HAU4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3), 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911912 |
Product Specific Information
Positive test controls include: U2OS, MCF7, SH-SY5Y, Mouse lung, Mouse testis, Mouse brain.
The target is typically localized in the cell membrane, cytoplasm, membrane raft, and nucleus.
Immunogen sequence:
PLSCFVDENT PISGTNGATC GQSSDPRLAE RRVRSQRHRN YMSRTHLHTP PDLPEGYEQR TTQQGQVYFL HTQTGVSTWH DPRVPRDLSN
Target Information
SMURF2 is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 conjugating enzyme to target substrates. It interacts with SMAD1, SMAD2, and SMAD7 to promote ubiquitination and proteasome-dependent degradation. SMURF2 enhances SMAD7 inhibitory activity and reduces SMAD2 transcriptional activity. Coexpression with SMAD1 leads to significant reduction in SMAD1 protein levels and a smaller decrease in SMAD2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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