Thermo Fisher Scientific Human MCL1 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 16 amino acids near the amino terminus of human Mcl-1.
PEP-0240 can be used as a blocking peptide with polyclonal antibody PA5-20122.

Target Information

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programming, differentiation, and maintenance of cell viability, but not proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and Bcl-2 share significant sequence homology.

Expression of MCL1 increases upon exposure of ML-1 cells to DNA-damaging agents (ionizing radiation, ultraviolet radiation, alkylating drugs) along with increases in GADD45 and Bax and a decrease in Bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements continued Bcl-2 expression in ML-1 cells undergoing differentiation.

Like Bcl-2, MCL1 promotes cell viability under conditions that otherwise cause apoptosis. It may inhibit apoptosis by heterodimerizing and neutralizing pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 is expressed in multiple cell types and is essential for embryogenesis and the development and maintenance of B and T lymphocytes. MCL1 exists as at least two distinct isoforms: MCL1L and MCL1S. Overexpression of MCL1S promotes cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.