Thermo Fisher Scientific Human MCL1 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 16 amino acids near the center of human Mcl-1.
PEP-0239 can be used as a blocking peptide with polyclonal antibody PA5-20121.

Target Information

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programming, differentiation, and maintenance of cell viability, but not proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and Bcl-2 share significant sequence homology.

Expression of MCL1 increases upon exposure of ML-1 cells to DNA damaging agents (e.g., ionizing radiation, ultraviolet radiation, alkylating drugs) along with increases in GADD45 and Bax and a decrease in Bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of Bcl-2 in ML-1 cells undergoing differentiation.

Like Bcl-2, MCL1 promotes cell viability under conditions that otherwise cause apoptosis. Although the mechanism by which MCL1 inhibits apoptosis is not fully known, it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak.

MCL1 was originally identified in differentiating myeloid cells but is expressed in multiple cell types. It is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms, MCL1L and MCL1S; overexpression of MCL1S promotes cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.