Thermo Fisher Scientific PKC theta Polyclonal Antibody
PKC theta 단백질을 인식하는 Thermo Fisher의 토끼 폴리클로날 항체로, Western blot 및 IHC(파라핀) 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, Human 및 Mouse에 반응합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | — |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 15–294 of Human PKC theta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2546565 |
Product Specific Information
- Recommended positive control: Jurkat
- Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (80%), Rhesus Monkey (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways governing differentiation, proliferation, gene expression, brain function, membrane transport, and cytoskeletal organization. PKC acts as a key regulator of cell cycle transitions (G1 and G2 checkpoints), with effects depending on activation timing and isozyme type.
At least 12 PKC isoforms have been identified in humans, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. PKC beta II is expressed in cardiac muscle, intestinal epithelium, kidney, and pancreas, and is associated with cell cycle progression and cancer proliferation.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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