Thermo Fisher Scientific Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody
AKT 단백질의 Tyr315, Tyr316, Tyr312 인산화 부위를 인식하는 폴리클로날 항체. Western blot 및 IHC(Paraffin) 실험에 적합. Human, Mouse, Rat 반응성. 고순도(>95%) 항원 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
- Publications: View 1 publication
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
- Publications: View reference
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyrosine 315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553727 |
Product Specific Information
- Detects endogenous AKT protein at a molecular weight of 60 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a ubiquitous serine/threonine kinase involved in diverse biological responses such as metabolism regulation, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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