Thermo Fisher Scientific His Tag Monoclonal Antibody (OTI2B5 (formerly 2B5)), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4000

-

Miscellaneous PubMed (Misc)

-

View 13 publications 13 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2B5 (formerly 2B5)

Immunogen

Anti-His monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4 mg/mL

Purification

Protein G

Storage buffer

PBS with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Synthetic peptide YPYDVPDYA conjugated to KLH

400 µg lyophilized powder. The lyophilized antibody can be reconstituted with 100 µL of ddH2O. The concentration after reconstitution will be 4 µg/µL.

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.