Thermo Fisher Scientific His tag Polyclonal Antibody
6x His tag를 인식하는 Thermo Fisher Scientific의 폴리클로날 항체입니다. Western blot에 최적화되어 있으며, 재조합 단백질 검출 및 정제 과정에 활용됩니다. Rabbit IgG 기반으로 제작되었으며, -20°C에서 보관 가능합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific His tag Polyclonal Antibody
Applications
- Western Blot (WB): 1:500–1:2,000 dilution
Publications
- Miscellaneous PubMed (Misc): 1 publication available
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1 mg/mL |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
Synthetic peptide sequence: HHHHHH
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of proteins lacking specific antibodies by fusing a known epitope to the recombinant protein.
His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to the strong and selective chelation of histidine residues to nickel ions.
His tag-specific antibodies are useful for detection or co-immunoprecipitation of tagged proteins.
Important Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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