Thermo Fisher Scientific His tag Polyclonal Antibody

Thermo Fisher Scientific His tag Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:2,000 dilution

Publications

• Miscellaneous PubMed (Misc): 1 publication available

Product Specifications

Product Specific Information

Synthetic peptide sequence: HHHHHH

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of proteins lacking specific antibodies by fusing a known epitope to the recombinant protein.
His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to the strong and selective chelation of histidine residues to nickel ions.
His tag-specific antibodies are useful for detection or co-immunoprecipitation of tagged proteins.

Important Notice

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.