Thermo Fisher Scientific Sox17 Monoclonal Antibody (OTI2F9), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

Flow Cytometry (Flow)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI2F9

Immunogen

Human recombinant protein fragment corresponding to amino acids 177-414 of human SOX17 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SOX17,uniProtId:Q9H6I2-1,ncbiNodeId:9606,antigenRange:177-414,antigenLength:414,antigenImageFileName:CF500045_SOX17_Q9H6I2-1_House_mouse.svg,antigenImageFileNamePDP:CF500045_SOX17_Q9H6I2-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

SOX17 (transcription factor SOX-17) belongs to the SRY-related HMB box family of transcription factors. It acts as a transcription regulator that binds target promoter DNA and bends the DNA. It binds to the sequences 5-AACAAT-3 or 5-AACAAAG-3. SOX17 modulates transcriptional regulation via WNT3A and inhibits Wnt signaling. It promotes degradation of activated CTNNB1. SOX17 plays an essential role in the regulation of embryonic development and is required for normal looping of the embryonic hear tube and for normal development of the definitive gut endoderm. Mutations in the gene can result in vesicoureteral reflux.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.