
Thermo Fisher Scientific CDC6 Polyclonal Antibody
CDC6 단백질 인식을 위한 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 4°C 보관, 습빙 상태로 배송됩니다. 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human cell division cycle 6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The CDC6 protein is highly similar to Saccharomyces cerevisiae Cdc6, which is essential for the initiation of DNA replication. It functions as a regulator in the early steps of DNA replication and localizes in the nucleus during G1 phase, translocating to the cytoplasm at the start of S phase. This translocation is regulated by phosphorylation via Cdks. Transcription of CDC6 is controlled by mitogenic signals through E2F-mediated transcriptional mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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