Thermo Fisher Scientific CD8 Monoclonal Antibody (49-31.1)
Mouse CD8 단백질을 인식하는 단클론 항체로 Flow Cytometry에 최적화됨. Protein G로 정제된 액상 형태이며 1 mg/mL 농도. PBS buffer에 0.02% sodium azide 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 1 µg/1 million cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG3, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 49-31.1 |
| Immunogen | 129/ReJ |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1073403 |
Product Specific Information
MA1-70076 detects CD8a in mouse samples.
MA1-70076 has been successfully used in flow cytometry procedures.
The MA1-70076 immunogen is 129/ReJ.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It plays an integral role in signal transduction, T cell differentiation, and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with T cell receptor (TCR) for MHC class I molecules.
CD8α is essential for binding to MHC-I and is also expressed on subsets of T cells, NK cells, monocytes, and dendritic cells as homodimers.
Engagement of MHC-I/peptide complexes triggers recruitment of Lck kinase, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs play a crucial role in pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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