Thermo Fisher Scientific CD186 (CXCR6) Monoclonal Antibody (DANID2), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1 µg/test
• Publications: View 1 publication

Product Specifications

Available Formats

• APC
• PE
• Request custom conjugation

Product Specific Information

Description:
This DANID2 monoclonal antibody recognizes mouse CD186 (CXCR6). It is suitable for flow cytometry on both native and paraformaldehyde-fixed cells.

Applications Reported:
Flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1.0 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize antibody titration for best performance.

Fluorochrome Information:
PerCP-eFluor™ 710 emits at 710 nm and is excited with a 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Verify instrument compatibility.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Performance may vary by clone; empirical testing recommended.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser

Target Information

CD186 (CXCR6) is a G protein-coupled chemokine receptor and one of the cellular receptors for HIV and SIV. Expressed by NKT cells, activated/memory T cells, and some plasma cells. In mice, liver NK cells (but not naïve or splenic NK cells) express CD186. Its ligand, CXCL16, is expressed by macrophages, dendritic cells, and liver endothelial cells. CD186 serves as a marker for NKT cell subsets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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