Thermo Fisher Scientific EB1 Polyclonal Antibody
EB1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용 가능. 인간 및 마우스 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 연구용으로만 사용.
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Thermo Fisher Scientific EB1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 245 and 268 of human End-binding protein 1 (EB1). Target Family: EB1, UniProt ID: Q15691-1, NCBI Node ID: 9606, Antigen Range: 245–268 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Predicted to react with Rat, Bovine, and Orangutan (based on 100% sequence identity)
Target Information
EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes, regulating microtubule dynamics and organization.
The MAPRE genes encode EB1 family proteins: MAPRE1 (EB1), MAPRE2 (RP1), and MAPRE3 (EBF3).
EB1 (MAPRE1) gene, located at 20q11.2, can be fused to MLL in pro-B acute lymphoblastic leukemia.
EB1 interacts with the APC tumor-suppressor protein and plays key roles in microtubule dynamics, cell polarity, and chromosome stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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