Thermo Fisher Scientific SMAD2/SMAD3 Polyclonal Antibody
SMAD2/SMAD3 단백질을 인식하는 Rabbit Polyclonal Antibody로, 인간·마우스·랫트 반응성. WB, IHC, ICC, ELISA 등 다양한 응용 가능. 액상 형태, 0.32 mg/mL 농도, Affinity Chromatography 정제. 연구용 전용 제품으로 세포 내 신호전달 연구에 적합.
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Thermo Fisher Scientific SMAD2/SMAD3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 150–270 of human SMAD2/SMAD3 (UniProt Q15796-1, P84022-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.32 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914229 |
Product Specific Information
- Positive test controls: 293T cells
- Localization: Cytoplasm, Nucleus
- Immunogen sequence:
EYAFNLKKDE VCVNPYHYQR VETPVLPPVL VPRHTEILTE LPPLDDYTHS IPENTNFPAG IEPQSNYIPE TPPPGYISED GETSDQQLNQ SMDTGSPAEL SPTTLSPVNH SLDLQPVTYS E
Target Information
Smad proteins are mammalian homologs of the Drosophila mothers against decapentaplegic (Mad) and act as downstream effectors of TGFβ/BMP signaling.
- Smad1 and Smad5: Effectors of BMP-2 and BMP-4
- Smad2 and Smad3: Involved in TGFβ and Activin-mediated growth modulation
- Smad4: Mediates activities through interaction with other Smad family members
- Smad6 and Smad7: Regulate Activin/TGFβ signaling by interfering with phosphorylation of other Smad proteins
Usage Notes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-120657_SMAD2SMAD3_Q15796-1_Rabbit.svg, PA5-120657_SMAD2SMAD3_Q15796-1_Rabbit_PDP.jpeg, PA5-120657_SMAD2SMAD3_P84022-1_Rabbit.svg, PA5-120657_SMAD2SMAD3_P84022-1_Rabbit_PDP.jpeg)
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