Thermo Fisher Scientific Wilms Tumor 1 (WT1) Recombinant Rabbit Monoclonal Antibody (WT1, 1434R)
Human WT1 단백질을 인식하는 Recombinant Rabbit Monoclonal 항체로, IHC(P)에서 1–2 µg/mL 농도로 사용 가능. HEK293 세포에서 발현되었으며, Protein A/G로 정제된 액상 형태. PBS(pH 7.4)에 보존제 없이 저장되며, 연구용으로만 사용 가능.
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Applications
Tested Application
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | WT1, 1434R |
| Immunogen | Recombinant human full-length WT1 protein (UniProt ID: P19544-1, amino acids 1–449) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells.
Ran GTPase is essential for nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly.
The cytoplasmic RanGAP protein establishes the RanGTP/RanGDP gradient across the nuclear envelope and associates with the outer nuclear membrane in metazoan and higher plant cells.
WT1 and WIT2 are identified as a second family of coiled-coil and transmembrane domain proteins (CC-TMD), distinct from the WIP family, necessary for RanGAP nuclear envelope association in root tip cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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