Thermo Fisher Scientific CD8 Monoclonal Antibody (FK18)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

FK18

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant with 1% FBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073406

Product Specific Information

The antibody reacts with CD8, the 33 kD T8 antigen present on the human cytotoxic/suppressor T-cell subset. It strongly blocks the cytolytic activity of T8-positive CTLs. It also reacts with a subpopulation of Rhesus monkey lymphocytes which is identical to the population of human T8 positive cells.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.