Thermo Fisher Scientific CD74 Monoclonal Antibody (5-329), PE, eBioscience
인간 CD74(MHC Class II 관련 불변사슬)을 인식하는 단클론 항체. Flow Cytometry용으로 최적화된 PE 결합 항체로, 5 µL/test 권장. B세포, 활성화 T세포 등에서의 CD74 발현 분석에 적합. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 5 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5-329 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10855028 |
Additional Formats:
Product Specific Information
Description:
This 5-329 monoclonal antibody recognizes human CD74 (MHC Class II-associated invariant chain), a 33–43 kDa nonpolymorphic type II membrane protein. CD74 is highly expressed on B cells, subsets of activated T cells, Langerhans cells, dendritic cells, and epithelial cells. It acts as a chaperone for MHC Class II (HLA-DR) proteins, aiding their transport to endocytic compartments during antigen presentation. CD74 interaction with CD44 and MIF is linked to NFκB and ERK activation. It also contributes to follicular B-cell maturation and marginal zone B-cell accumulation, and interacts with CXCR2 and CXCR4.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg)/test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB). The DRA gene consists of 5 exons encoding leader peptide, extracellular domains, transmembrane domain, and cytoplasmic tail. DRA lacks polymorphisms in the peptide-binding region and pairs with DRB1, DRB3, DRB4, and DRB5. DRB1 allelic polymorphisms determine peptide-binding specificity and are important in transplantation typing. HLA-DR is primarily expressed on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes. MHC class II molecules (DR, DQ, DP) present processed peptides to T-helper cells, initiating immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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