Thermo Fisher Scientific CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscience
마우스 CD34 단백질을 인식하는 RAM34 단클론 항체로, Alexa Fluor 700으로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 줄기세포 표지 및 분리 연구에 활용됩니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 20 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | - | View 10 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 84 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Goat, Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RAM34 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696/719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_493998 |
Additional Formats:
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Biotin
- eFluor 450
- eFluor 660
- FITC
- Request custom conjugation
Product Specific Information
The RAM34 monoclonal antibody reacts with mouse CD34 (mucosialin). It is used to detect CD34⁺Sca-1⁺c-Kit⁺ cells. CD34 is a 90–120 kDa sialomucin family member expressed on capillary endothelial cells, bone marrow stroma, and a subset of mouse bone marrow cells. RAM34 enables purification of mouse hematopoietic stem cells (HSC) to near homogeneity.
CD34 on endothelial cells acts as a ligand for CD62L and plays a role in adhesion. Co-staining of mouse bone marrow cells with lineage markers (CD3, CD11b, Ly6G, TER-119, CD45R/B220) identifies RAM34⁺ cells with low or undetectable lineage marker expression.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometry using ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
- Recommended incubation: 90 minutes for optimal signal-to-noise ratio
- Excitation/Emission: 633–647 nm / 723 nm (Red Laser)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD34 is a monomeric, highly glycosylated (111–115 kDa) surface protein expressed on various stem cell populations. It functions as a receptor involved in adhesion, differentiation, and proliferation of hematopoietic stem and progenitor cells. CD34 expression indicates a developmental state associated with altered adhesion and differentiation potential. It may serve as a scaffold for binding glycans and lectins, mediating stem cell attachment to the bone marrow matrix or stromal cells. The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction.
Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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