Thermo Fisher Scientific CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Biotin
• eFluor 450
• eFluor 660
• FITC
• Request custom conjugation

Product Specific Information

The RAM34 monoclonal antibody reacts with mouse CD34 (mucosialin). It is used to detect CD34⁺Sca-1⁺c-Kit⁺ cells. CD34 is a 90–120 kDa sialomucin family member expressed on capillary endothelial cells, bone marrow stroma, and a subset of mouse bone marrow cells. RAM34 enables purification of mouse hematopoietic stem cells (HSC) to near homogeneity.

CD34 on endothelial cells acts as a ligand for CD62L and plays a role in adhesion. Co-staining of mouse bone marrow cells with lineage markers (CD3, CD11b, Ly6G, TER-119, CD45R/B220) identifies RAM34⁺ cells with low or undetectable lineage marker expression.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry using ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• Recommended incubation: 90 minutes for optimal signal-to-noise ratio
• Excitation/Emission: 633–647 nm / 723 nm (Red Laser)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD34 is a monomeric, highly glycosylated (111–115 kDa) surface protein expressed on various stem cell populations. It functions as a receptor involved in adhesion, differentiation, and proliferation of hematopoietic stem and progenitor cells. CD34 expression indicates a developmental state associated with altered adhesion and differentiation potential. It may serve as a scaffold for binding glycans and lectins, mediating stem cell attachment to the bone marrow matrix or stromal cells. The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction.

Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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