Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), Alexa Fluor 700, eBioscience
인간 CD34 항원을 인식하는 4H11 단클론 항체로, Alexa Fluor 700 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 조혈모세포 및 내피세포 연구에 활용됩니다. 고순도 친화 크로마토그래피 정제 및 안정한 PBS 완충액에 보존됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 54 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H11 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815231 |
Additional Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The 4H11 monoclonal antibody reacts with human CD34 (mucosialin). CD34 belongs to a family including endoglycan and podocalyxin, which are transmembrane proteins with heavily glycosylated extracellular domains. CD34 was first identified on hematopoietic progenitors and is widely used as a marker for cells capable of engraftment. It is also expressed on mesenchymal stem cells, tumor cell lines, and vascular endothelium.
Epitopes of CD34 are classified as I, II, or III based on enzymatic cleavage sensitivity. The 4H11 antibody belongs to class III, reacting with a protein epitope.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test in a final volume of 100 µL. Applicable cell numbers: 10⁵–10⁸ cells/test.
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and can be excited by the red laser (633–647 nm). Recommended optical setup: 685 LP mirror and 710/20 bandpass filter.
Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on various stem cell populations. It functions as a stem cell marker, potentially involved in receptor-mediated endocytosis, adhesion, differentiation, and proliferation regulation. CD34 may mediate stem cell attachment to the bone marrow matrix or stromal cells and act as a scaffold for glycan attachment, facilitating lectin binding. The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction.
Associated Diseases:
Dermatofibrosarcoma, Neurofibroma
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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