Thermo Fisher Scientific Phospho-IGF2R (Ser2484) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Product Specifications

Product Specific Information

Antibody detects endogenous levels of IGF2R only when phosphorylated at Ser2484.

Target Information

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are transported within the cell by cation-independent mannose 6-phosphate receptors (CI-MPR, ~300 kDa).
These receptors participate in two interconnected cycles:

1. Biosynthetic cycle – Transports newly synthesized lysosomal enzymes from the trans-Golgi network to late endosomes or early lysosomes.
2. Endocytic cycle – Transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes.

MPRs cycle between these compartments approximately every 3 hours.
The majority of MPRs are found in late endosomes, fewer in the trans-Golgi network, and about 10% at the cell surface.
In addition to man6P binding, MPRs have a separate binding site for type II insulin-like growth factor (IGF-II) and can bind both ligands simultaneously.
A soluble, truncated form (~240 kDa) representing the extracellular domain circulates in serum and retains ligand-binding capacity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.