Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (2D12), PE
6-Nitrotryptophan 변형 단백질을 특이적으로 인식하는 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. PE 형광 표지로 손쉬운 검출. 1 mg/mL 액상 형태로 제공되며 4°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D12 |
| Immunogen | Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- A 1:1,000 dilution of MA5-45820 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotryptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
- Specific for 6-Nitrotryptophan modified proteins.
- Does not cross-react with 7-Ketocholesterol modified proteins.
Target Information
6-Nitrotryptophan (6-NO₂-Trp) is one of the most stable and major ONOO⁻-modified amino acids. Evidence suggests that nitrotryptophan plays a significant role in cellular regulation under nitrosative stress. Reactive nitrogen species (RNS) are formed in diseases such as atherosclerosis, inflammation, cancer, and neurodegenerative disorders. Peroxynitrite (ONOO⁻), a potent biological oxidant formed from nitric oxide (NO) and superoxide (O₂⁻), can induce nitration of aromatic amino acids (Tyr, Trp, Phe, His), leading to modulation of protein function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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